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The time-resolved immunofluorometric assay （TRIFA） was used to detect the hepatitis B virus markers, the FQ-PCR method was used to measure HBV-DNA. 方法选择新八院乙肝患者的血清，应用时间分辨免疫荧光分析法（TRIFA）测定乙肝病毒标志物的含量；