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Later, Using modified Pallo method to separate HM involves Humin linked to iron (HMi), Humin linked to clay(HMc), Humin indissoluble (HMr). 采用Pallo修改法对所得的HM分组，组分包括铁结合胡敏素(HMi)、粘粒结合胡敏素(HMc)和不溶性胡敏素(HMr)。