bicistronic

基本解释

Results The bicistronic eukaryotic expression plasmid was corrected and can co - express human BMP -2 and VEGF165 mRNA in vitro.
该重组质粒能在体外同时表达BMP2及VEGF165 mRNA。
Results showed that IRES was superior to SV40 promoter for guarantee of genes co expression in bicistronic retrovirus mediated gene therapy.
结果表明:在双基因逆转录病毒载体介导的小鼠骨髓细胞的基因转导中,IRES与内部SV40启动子相比,更能保证双基因的共同表达。
ALR gene was also inserted to bicistronic plasmid p! RES2-EGFP to produce ALR, EGFP gene bicistronic eukaryotic expression vector (pIRES-EGFP/ALR).
ALR和EGFP基因双顺反子表达载体，pIRES-EGFP/ALR。
Cytokine expressing plasmids pcDNA3/GM-CSF and pcDNA3.1(-)/IL-4 and , bicistronic plasmid pGM-CSF/pTPA-E were constructed.
构建小鼠细胞因子表达质粒pcDNA3/GM-CSF和pcDNA3.;1(-)/IL-4，以及双表达质粒pGM-CSF/pTPA-E;用细胞因子表达质粒分别与VR1020/E共同免疫小鼠，分析细胞因子质粒对AMA1 DNA免疫的调节作用。
Mouse marrow stromal cells (MSCs) was transfected with this bicistronic eukaryotic expression plasmid using lipofecarmin reagent The expression of BMP -2 and VEGF165 were detected by RT - PCR.
进行酶切分析及序列测定后,用脂质体体外转染小鼠骨髓基质细胞,RT-PCR检测BMP2及VEGF165的表达。结果:核酸序列测定证实重组质粒构建正确;